Journal: Circulation Research

Article Title: miR33 Inhibition Overcomes Deleterious Effects of Diabetes Mellitus on Atherosclerosis Plaque Regression in Mice

doi: 10.1161/circresaha.115.304164

Figure Lengend Snippet: Figure 2. α-miR33 treatment restores regression in diabetic mice. Aortic roots from baseline and the regression groups were sectioned, fixed, and stained for CD68 (A) and collagen (B). Representative pictures of CD68 immunostaining (A, magnification ×20) and picrosirius red staining (B, under white and polarized light) of collagen (magnification ×10) are shown for each group. The areas of the plaques occupied by CD68+ cells and collagen (the latter as detected by polarized light) were quantified by Image Pro Plus Software and displayed in the graphs. Results are expressed as the percentage of plaque area. ^P≤0.05 vs baseline, #P≤0.05, ###P≤0.001 vs con α-miR normoglycemic; ***P≤0.001 vs α-miR33 diabetic.

Article Snippet: For immunostaining of CD68 (macrophage marker), slides were fixed in 100% acetone and exposed to primary anti-CD68 antibody (Serotec), followed by biotinylated secondary antibody (Vector Laboratories), with visualization using a Vectastain ABC kit (Vector Laboratories).

Techniques: Staining, Immunostaining, Software

Journal: Scientific Reports

Article Title: Dietary wheat amylase trypsin inhibitors promote features of murine non-alcoholic fatty liver disease

doi: 10.1038/s41598-019-53323-x

Figure Lengend Snippet: ATI feeding increases liver macrophage numbers and their M1- vs M2-type polarization. ( A – C ) Immunohistochemistry and quantitative morphometry for CD68 and YM-1 positive cells (original magnification 40x). ( D ) Ratio of total (CD68+) vs M2-type (Ym-1+) macrophages. ( E ) CD11b+ F4/80+ macrophage subset (% of CD45 positive total immune cells) as determined by FACS analysis. Comparisons by ANOVA; data are means ± SEM for 10 representative sections per mouse and 7–10 mice per group; *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Tissue was blocked with 5% normal donkey serum, and subsequently incubated with primary antibodies of to CD68 (1:100, Biozol, clone: FA-11), CD86 (1:100, Abcam, cat no: ab119857), and MHC-II (1:100, Abcam, Cat no: 180779) for 2 hrs at room temperature and finally incubated with respective Alexa-flour 488 labelled secondary antibodies.

Techniques: Immunohistochemistry

Journal: Scientific Reports

Article Title: Dietary wheat amylase trypsin inhibitors promote features of murine non-alcoholic fatty liver disease

doi: 10.1038/s41598-019-53323-x

Figure Lengend Snippet: ATI feeding increases hepatic pro-inflammatory and macrophage M1- vs M2-type gene expression. ( A – F ) Hepatic transcript levels of cd68, tnfa, il1b, il6, arg1 and ym1. Comparisons by ANOVA; data are expressed as means ± SEM for 7–10 mice per group; *p < 0.05, **p < 0.01.

Article Snippet: Tissue was blocked with 5% normal donkey serum, and subsequently incubated with primary antibodies of to CD68 (1:100, Biozol, clone: FA-11), CD86 (1:100, Abcam, cat no: ab119857), and MHC-II (1:100, Abcam, Cat no: 180779) for 2 hrs at room temperature and finally incubated with respective Alexa-flour 488 labelled secondary antibodies.

Techniques: Gene Expression

Journal: Scientific Reports

Article Title: Dietary wheat amylase trypsin inhibitors promote features of murine non-alcoholic fatty liver disease

doi: 10.1038/s41598-019-53323-x

Figure Lengend Snippet: Nutritional ATI promote central adipose tissue inflammation. ( A ) Crown like structures (CLS = accumulation of macrophages) in CD68+ stained sections of epididymal adipose tissue in the 4 experimental groups (original magnification 40x), the number of CD68+ CLS as determined by morphometry, and epididymal fat as % of body weight. ( B ) fat weights, and ( C ) transcript levels of cd68, il6 and il1b. Comparisons by ANOVA; data are means ± SEM for 10 representative sections per mouse and 7–10 mice per group; *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Tissue was blocked with 5% normal donkey serum, and subsequently incubated with primary antibodies of to CD68 (1:100, Biozol, clone: FA-11), CD86 (1:100, Abcam, cat no: ab119857), and MHC-II (1:100, Abcam, Cat no: 180779) for 2 hrs at room temperature and finally incubated with respective Alexa-flour 488 labelled secondary antibodies.

Techniques: Staining

Journal: Scientific Reports

Article Title: Dietary wheat amylase trypsin inhibitors promote features of murine non-alcoholic fatty liver disease

doi: 10.1038/s41598-019-53323-x

Figure Lengend Snippet: ATI feeding increases intestinal macrophage and dendritic cell activation and maturation. ( A–C ) CD68, CD86 and MCH-II expressing cells in the terminal ileum; scale bar: 100 and 50 µm. ( D ) Morphometric quantification of CD68, CD86 and MHC-II positive cells. ( E ) Transcript levels of il1b, tnfα and il6. Comparisons by ANOVA; data are expressed as means ± SEM of 6 mice per group and 5 representative sections per mouse; *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Tissue was blocked with 5% normal donkey serum, and subsequently incubated with primary antibodies of to CD68 (1:100, Biozol, clone: FA-11), CD86 (1:100, Abcam, cat no: ab119857), and MHC-II (1:100, Abcam, Cat no: 180779) for 2 hrs at room temperature and finally incubated with respective Alexa-flour 488 labelled secondary antibodies.

Techniques: Activation Assay, Expressing

Journal: Journal of Virology

Article Title: Brain Large Artery Lymphocytic Inflammation and Human Immunodeficiency Virus-Related Brain Arterial Remodeling

doi: 10.1128/JVI.00081-18

Figure Lengend Snippet: Intima CD3 + /CD68 + cell phenotypes and markers of brain arterial remodeling a

Article Snippet: Briefly, anti-CD68 + antibody (primary, 1:100, catalog number M0814, from Dako Corp., Carpinteria, CA; secondary, biotinylated horse anti-mouse IgG antibody, catalog number BA-2000, from Vector Laboratories, Burlingame, CA) binding to arteries was counterstained with hematoxylin and visualized using the diaminobenzidine kit ( 10 ).

Techniques: Isolation

Journal: Cancer Microenvironment

Article Title: Tumor-Associated T-Lymphocytes and Macrophages are Decreased in Endometrioid Endometrial Carcinoma with MELF-Pattern Stromal Changes

doi: 10.1007/s12307-018-0213-5

Figure Lengend Snippet: Infiltration of macrophages and dendritic cells in zone of invasion. Immunochistocemistry for CD68: CD68+ tumor-associated macrophages infiltrate lumen of the gland and cancer stroma (arrows) in group with MELF-pattern (a); dense infiltrate by CD68+ tumor-associated macrophages (arrows) of cancer stroma in group without MELF-pattern (b); Immunochistocemistry for S100: infiltration of cancer glands and stroma (arrows) by weak infiltrates presented by few S100+ tumor-associated dendritic cells in both groups (c-d). Magnification: ×200. Counterstain: Hematoxylin

Article Snippet: Primary antibodies used in this study include the following: ready-to-use monoclonal mouse anti-CD68 (TAM), anti-CD3 (TAT), anti-CD20 (TAB), anti-CD57 (NK), and ready-to-use polyclonal rabbit anti-S100 (DC) (Diagnostic Biosystems).

Techniques:

Journal: Cancer Microenvironment

Article Title: Tumor-Associated T-Lymphocytes and Macrophages are Decreased in Endometrioid Endometrial Carcinoma with MELF-Pattern Stromal Changes

doi: 10.1007/s12307-018-0213-5

Figure Lengend Snippet: Forest plot represents odds ratio and 95% confidence interval of the association between infiltration by each type of immune cell and MELF-pattern stromal changes. The result of analysis showed statistically significant association between decrease of TAT and TAM and presence of MELF-pattern. Abbreviations: CD3 – tumor-associated CD3+ T-lymphocytes; CD20 – tumor-associated CD20+ B-lymphocytes; CD57 – tumor-associated CD57+ NK-lymphocytes; CD68 – tumor-associated CD68+ macrophages; S100+ − tumor-associated S100+ dendritic cells

Article Snippet: Primary antibodies used in this study include the following: ready-to-use monoclonal mouse anti-CD68 (TAM), anti-CD3 (TAT), anti-CD20 (TAB), anti-CD57 (NK), and ready-to-use polyclonal rabbit anti-S100 (DC) (Diagnostic Biosystems).

Techniques:

Journal: Stem cell research & therapy

Article Title: Subretinal microglia support donor photoreceptor survival in rd1 mice.

doi: 10.1186/s13287-024-04052-0

Figure Lengend Snippet: Fig. 2 Macrophage and microglia detection after transplantation. A. In rd1 mice, macrophages (CD68+) were observed in the choroid when donor cells either survived (left) or died (middle). In wild-type mice, dead donor cells were engulfed by macrophages (right). Scale bar = 10 μm. B. In trans-scleral injection group, while microglial cells surrounded surviving donor cells in rd1 mice (i), they could hardly be detected around dead cell debris (ii). In wild-type mice, microglia/macrophages engulfed dead donor cells (iii). Following trans-vitreous injection, both dead (arrows) and surviving donor cells (arrowheads) were found in the subretinal space. While macrophages/microglia engulfed the dead cells (arrows), microglia were not close to surviving cells (iv). C. A Comparison of chemokines expression between wild-type and rd1 mice showed a significant increase in the mRNA levels of ccl2, ccl3 and cxcl2 in rd1 mice. D. The comparison of cytokine levels between wild-type and rd1 mice showed no significant difference. E. No significant differences were found in oxidative stress factors between wild-type and rd1 mice. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The following primary antibodies were used in the study: CD68 (1:400,#97778, Cell Signaling Technology), Iba1 (1:200, #019-19741, Wako), Galectin-3 (1:200, #sc-53127, Santa Cruz), F4/80(1:400, #71299, Cell Signaling Technology), CD11b (1:200, 101,209, Biolegend).

Techniques: Transplantation Assay, Injection, Comparison, Expressing