Journal: Journal of Virology

Article Title: Brain Large Artery Lymphocytic Inflammation and Human Immunodeficiency Virus-Related Brain Arterial Remodeling

doi: 10.1128/JVI.00081-18

Figure Lengend Snippet: Intima CD3 + /CD68 + cell phenotypes and markers of brain arterial remodeling a

Article Snippet: Briefly, anti-CD68 + antibody (primary, 1:100, catalog number M0814, from Dako Corp., Carpinteria, CA; secondary, biotinylated horse anti-mouse IgG antibody, catalog number BA-2000, from Vector Laboratories, Burlingame, CA) binding to arteries was counterstained with hematoxylin and visualized using the diaminobenzidine kit ( 10 ).

Techniques: Isolation

Journal: Cancer Microenvironment

Article Title: Tumor-Associated T-Lymphocytes and Macrophages are Decreased in Endometrioid Endometrial Carcinoma with MELF-Pattern Stromal Changes

doi: 10.1007/s12307-018-0213-5

Figure Lengend Snippet: Infiltration of macrophages and dendritic cells in zone of invasion. Immunochistocemistry for CD68: CD68+ tumor-associated macrophages infiltrate lumen of the gland and cancer stroma (arrows) in group with MELF-pattern (a); dense infiltrate by CD68+ tumor-associated macrophages (arrows) of cancer stroma in group without MELF-pattern (b); Immunochistocemistry for S100: infiltration of cancer glands and stroma (arrows) by weak infiltrates presented by few S100+ tumor-associated dendritic cells in both groups (c-d). Magnification: ×200. Counterstain: Hematoxylin

Article Snippet: Primary antibodies used in this study include the following: ready-to-use monoclonal mouse anti-CD68 (TAM), anti-CD3 (TAT), anti-CD20 (TAB), anti-CD57 (NK), and ready-to-use polyclonal rabbit anti-S100 (DC) (Diagnostic Biosystems).

Techniques:

Journal: Cancer Microenvironment

Article Title: Tumor-Associated T-Lymphocytes and Macrophages are Decreased in Endometrioid Endometrial Carcinoma with MELF-Pattern Stromal Changes

doi: 10.1007/s12307-018-0213-5

Figure Lengend Snippet: Forest plot represents odds ratio and 95% confidence interval of the association between infiltration by each type of immune cell and MELF-pattern stromal changes. The result of analysis showed statistically significant association between decrease of TAT and TAM and presence of MELF-pattern. Abbreviations: CD3 – tumor-associated CD3+ T-lymphocytes; CD20 – tumor-associated CD20+ B-lymphocytes; CD57 – tumor-associated CD57+ NK-lymphocytes; CD68 – tumor-associated CD68+ macrophages; S100+ − tumor-associated S100+ dendritic cells

Article Snippet: Primary antibodies used in this study include the following: ready-to-use monoclonal mouse anti-CD68 (TAM), anti-CD3 (TAT), anti-CD20 (TAB), anti-CD57 (NK), and ready-to-use polyclonal rabbit anti-S100 (DC) (Diagnostic Biosystems).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: The Activation of the Microglial NLRP3 Inflammasome Is Involved in Tuberous Sclerosis Complex-Related Neuroinflammation

doi: 10.3390/ijms26157244

Figure Lengend Snippet: Detection of HMC3 cell activation and ROS levels. ( A ) The mRNA level of AIF1 was elevated in the TSC2 KD group. ( B , C ) AIF1 fluorescence intensity was greater in TSC2 KD HMC3 cells than in control HMC3 cells. ( D ) The mRNA level of CD68 was elevated in the TSC2 KD group. ( E , F ) The CD68 fluorescence intensity of TSC2 KD HMC3 cells was greater than that of control cells. ( G , H ) The intensity of ROS fluorescence in TSC2 KD HMC3 cells was greater than that in control cells. DHE: Dihydroethidium; N = 3; * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The cells were subsequently treated with reagents containing 0.5% Triton X-100 and 5% fetal bovine serum for 30 min. Anti-AIF1 primary antibody (recombinant rabbit monoclonal, 1:500; HUABIO, Hangzhou, China) and anti-CD68 primary antibody (mouse monoclonal, 1:500; Proteintech, Wuhan, China) were incubated at 4 °C overnight.

Techniques: Activation Assay, Fluorescence, Control

Journal: International Journal of Molecular Sciences

Article Title: The Activation of the Microglial NLRP3 Inflammasome Is Involved in Tuberous Sclerosis Complex-Related Neuroinflammation

doi: 10.3390/ijms26157244

Figure Lengend Snippet: The effect of rapamycin intervention on HMC3 cells was detected. ( A – C ) Immunofluorescence assays revealed that AIF1 expression was downregulated in the TSC2 KD + Rapa group and the control + Rapa group and that CD68 expression was downregulated in the TSC2 KD + Rapa group. ( D ) RT-qPCR revealed a decrease in AIF1 mRNA levels in the TSC2 KD + Rapa group and the control + Rapa group and a decrease in CD68 mRNA levels in the TSC2 KD + Rapa group. ( E , F ) The level of ROS was significantly lower in the TSC2 KD + Rapa group than in the control + Rapa group. Rapa: rapamycin; N = 3; * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The cells were subsequently treated with reagents containing 0.5% Triton X-100 and 5% fetal bovine serum for 30 min. Anti-AIF1 primary antibody (recombinant rabbit monoclonal, 1:500; HUABIO, Hangzhou, China) and anti-CD68 primary antibody (mouse monoclonal, 1:500; Proteintech, Wuhan, China) were incubated at 4 °C overnight.

Techniques: Immunofluorescence, Expressing, Control, Quantitative RT-PCR

Journal: Circulation Research

Article Title: miR33 Inhibition Overcomes Deleterious Effects of Diabetes Mellitus on Atherosclerosis Plaque Regression in Mice

doi: 10.1161/circresaha.115.304164

Figure Lengend Snippet: Figure 2. α-miR33 treatment restores regression in diabetic mice. Aortic roots from baseline and the regression groups were sectioned, fixed, and stained for CD68 (A) and collagen (B). Representative pictures of CD68 immunostaining (A, magnification ×20) and picrosirius red staining (B, under white and polarized light) of collagen (magnification ×10) are shown for each group. The areas of the plaques occupied by CD68+ cells and collagen (the latter as detected by polarized light) were quantified by Image Pro Plus Software and displayed in the graphs. Results are expressed as the percentage of plaque area. ^P≤0.05 vs baseline, #P≤0.05, ###P≤0.001 vs con α-miR normoglycemic; ***P≤0.001 vs α-miR33 diabetic.

Article Snippet: For immunostaining of CD68 (macrophage marker), slides were fixed in 100% acetone and exposed to primary anti-CD68 antibody (Serotec), followed by biotinylated secondary antibody (Vector Laboratories), with visualization using a Vectastain ABC kit (Vector Laboratories).

Techniques: Staining, Immunostaining, Software

Journal: Scientific Reports

Article Title: Dietary wheat amylase trypsin inhibitors promote features of murine non-alcoholic fatty liver disease

doi: 10.1038/s41598-019-53323-x

Figure Lengend Snippet: ATI feeding increases liver macrophage numbers and their M1- vs M2-type polarization. ( A – C ) Immunohistochemistry and quantitative morphometry for CD68 and YM-1 positive cells (original magnification 40x). ( D ) Ratio of total (CD68+) vs M2-type (Ym-1+) macrophages. ( E ) CD11b+ F4/80+ macrophage subset (% of CD45 positive total immune cells) as determined by FACS analysis. Comparisons by ANOVA; data are means ± SEM for 10 representative sections per mouse and 7–10 mice per group; *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Tissue was blocked with 5% normal donkey serum, and subsequently incubated with primary antibodies of to CD68 (1:100, Biozol, clone: FA-11), CD86 (1:100, Abcam, cat no: ab119857), and MHC-II (1:100, Abcam, Cat no: 180779) for 2 hrs at room temperature and finally incubated with respective Alexa-flour 488 labelled secondary antibodies.

Techniques: Immunohistochemistry

Journal: Scientific Reports

Article Title: Dietary wheat amylase trypsin inhibitors promote features of murine non-alcoholic fatty liver disease

doi: 10.1038/s41598-019-53323-x

Figure Lengend Snippet: ATI feeding increases hepatic pro-inflammatory and macrophage M1- vs M2-type gene expression. ( A – F ) Hepatic transcript levels of cd68, tnfa, il1b, il6, arg1 and ym1. Comparisons by ANOVA; data are expressed as means ± SEM for 7–10 mice per group; *p < 0.05, **p < 0.01.

Article Snippet: Tissue was blocked with 5% normal donkey serum, and subsequently incubated with primary antibodies of to CD68 (1:100, Biozol, clone: FA-11), CD86 (1:100, Abcam, cat no: ab119857), and MHC-II (1:100, Abcam, Cat no: 180779) for 2 hrs at room temperature and finally incubated with respective Alexa-flour 488 labelled secondary antibodies.

Techniques: Gene Expression

Journal: Scientific Reports

Article Title: Dietary wheat amylase trypsin inhibitors promote features of murine non-alcoholic fatty liver disease

doi: 10.1038/s41598-019-53323-x

Figure Lengend Snippet: Nutritional ATI promote central adipose tissue inflammation. ( A ) Crown like structures (CLS = accumulation of macrophages) in CD68+ stained sections of epididymal adipose tissue in the 4 experimental groups (original magnification 40x), the number of CD68+ CLS as determined by morphometry, and epididymal fat as % of body weight. ( B ) fat weights, and ( C ) transcript levels of cd68, il6 and il1b. Comparisons by ANOVA; data are means ± SEM for 10 representative sections per mouse and 7–10 mice per group; *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Tissue was blocked with 5% normal donkey serum, and subsequently incubated with primary antibodies of to CD68 (1:100, Biozol, clone: FA-11), CD86 (1:100, Abcam, cat no: ab119857), and MHC-II (1:100, Abcam, Cat no: 180779) for 2 hrs at room temperature and finally incubated with respective Alexa-flour 488 labelled secondary antibodies.

Techniques: Staining

Journal: Scientific Reports

Article Title: Dietary wheat amylase trypsin inhibitors promote features of murine non-alcoholic fatty liver disease

doi: 10.1038/s41598-019-53323-x

Figure Lengend Snippet: ATI feeding increases intestinal macrophage and dendritic cell activation and maturation. ( A–C ) CD68, CD86 and MCH-II expressing cells in the terminal ileum; scale bar: 100 and 50 µm. ( D ) Morphometric quantification of CD68, CD86 and MHC-II positive cells. ( E ) Transcript levels of il1b, tnfα and il6. Comparisons by ANOVA; data are expressed as means ± SEM of 6 mice per group and 5 representative sections per mouse; *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Tissue was blocked with 5% normal donkey serum, and subsequently incubated with primary antibodies of to CD68 (1:100, Biozol, clone: FA-11), CD86 (1:100, Abcam, cat no: ab119857), and MHC-II (1:100, Abcam, Cat no: 180779) for 2 hrs at room temperature and finally incubated with respective Alexa-flour 488 labelled secondary antibodies.

Techniques: Activation Assay, Expressing